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FlowPRA™ Single Antigen  

Practical for identifying acceptable antigens prior to a solid organ transplant and for pre- and post-transplant screening, the FlowPRA Single Antigen products were created to profile high PRA patients using flow cytometry. Each bead is designed to react specifically to HLA Class I and HLA Class II antibodies, distinguished by different fluorescent properties leaving no concern for cross reactivity.

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Antigen Coated Beads

The collection of FlowPRA reagents in Class I and Class II consists of a pool of 32 beads, separated into 4 groups, each coated with different antigens. Control beads are also included to demonstrate test reliability and inclusion of common HLA antigens provides optimal profiling for high PRA patients. Additionally, 6 supplemental groups of Class I single antigen beads offers extra specificities for antibody screening.

 

Features & Benefits

  • Quickly identifies Class I and Class II HLA antibodies in high PRA patients
  • Identifies negative or safe antigens, even for high PRA patients
  • Confirms specificity by replacing dilution and absorption as strategies
  • Clarifies ambiguous results
  • Clarifies analysis of antibodies reactive to one or several dominant epitopes

 

Regulatory

For In Vitro Diagnostic Use.
 

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Showing 10 17 of 17 Products
Catalog #
Product Name
Format
Amount
FL1HD
FlowPRA Single Antigen HLA Class I
HLA-A, B
10 tests
Full Specification
HLA Class I
Product Name FlowPRA Single Antigen HLA Class I
Format HLA-A, B
Regulatory For In Vitro Diagnostic Use.
Use To identify HLA Class I IgG antibody specificities in high PRA sera by flow cytometry. A panel composed of 32 microbeads coated with purified HLA Class I single or fractional antigens divided into 4 groups for a panel typing of A and B loci. Each group contains one control bead not coated with antigen.
Package Includes 4 vials (Groups, 1, 2, 3. 4) FlowPRA Single Antigen Class I Beads (Cat.# FL1HDBD1, FL1HDBD2, FL1HDBD3, FL1HDBD4) 60 µl vials each vial, 5 µl per test, 10X wash buffer (Cat.# FL-BF) 26 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-AB) 60 µl vial
Shipping Considerations Dry ice
Storage Temperature -80° to -65° C or below
Amount 10 tests
Detection Method Flow Cytometric
Technique ELISA
Typing Resolution High
Target Group 1: A*01:01, A*02:01, A*03:01, B*49:01, A25:01, A*29:01, A*30:01, A*26:01, B*49:01
Group 2: A11:01, A*23:01, A*24:02, A*31:01, A32:01, A33:01, A*34:01, A*68:01
Group 3: B*13:01, B*15:01, B*18:01, B*35:01, B*10:01, B*45:01, B*44:02, B*45:01
Group 4: B*07:02, B*08:01, B*14:02, B*27:05, B*38:01, B*52:01, B*55:01, B57:01

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    FL1HD01
    FlowPRA Single Antigen HLA Class I - Group 1
    HLA-A, B
    10 tests
    Full Specification
    HLA Class I
    Product Name FlowPRA Single Antigen HLA Class I - Group 1
    Format HLA-A, B
    Regulatory For In Vitro Diagnostic Use.
    Use To identify HLA Class I antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of A and B loci, and one control bead that is not coated with antigen.
    Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
    Shipping Considerations Dry ice
    Storage Temperature -80° to -65° C or below
    Amount 10 tests
    Detection Method Flow Cytometric
    Technique ELISA
    Typing Resolution High
    Target Antigens: A*01:01, A*02:01, A*03:01, B*49:01, A25:01, A*29:01, A*30:01, A*26:01, B*49:01

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      FL1HD02
      FlowPRA Single Antigen HLA Class I - Group 2
      HLA-A
      10 tests
      Full Specification
      HLA Class I
      Product Name FlowPRA Single Antigen HLA Class I - Group 2
      Format HLA-A
      Regulatory For In Vitro Diagnostic Use.
      Use To identify HLA Class I antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of A locus, and one control bead that is not coated with antigen.
      Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
      Shipping Considerations Dry ice
      Storage Temperature -80° to -65° C or below
      Amount 10 tests
      Detection Method Flow Cytometric
      Technique ELISA
      Typing Resolution High
      Target Antigens: A11:01, A*23:01, A*24:02, A*31:01, A32:01, A33:01, A*34:01, A*68:01

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        FL1HD03
        FlowPRA Single Antigen HLA Class I - Group 3
        HLA-B
        10 tests
        Full Specification
        HLA Class I
        Product Name FlowPRA Single Antigen HLA Class I - Group 3
        Format HLA-B
        Regulatory For In Vitro Diagnostic Use.
        Use To identify HLA Class I antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of B locus, and one control bead that is not coated with antigen.
        Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
        Shipping Considerations Dry ice
        Storage Temperature -80° to -65° C or below
        Amount 10 tests
        Detection Method Flow Cytometric
        Technique ELISA
        Typing Resolution High
        Target Antigens: B*13:01, B*15:01, B*18:01, B*35:01, B*10:01, B*45:01, B*44:02, B*45:01

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          FL1HD04
          FlowPRA Single Antigen HLA Class I - Group 4
          HLA-B
          10 tests
          Full Specification
          HLA Class I
          Product Name FlowPRA Single Antigen HLA Class I - Group 4
          Format HLA-B
          Regulatory For In Vitro Diagnostic Use.
          Use To identify HLA Class I antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of B locus, and one control bead that is not coated with antigen.
          Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
          Shipping Considerations Dry ice
          Storage Temperature -80° to -65° C or below
          Amount 10 tests
          Detection Method Flow Cytometric
          Technique ELISA
          Typing Resolution High
          Target Antigens: B*07:02, B*08:01, B*14:02, B*27:05, B*38:01, B*52:01, B*55:01, B57:01

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            FL1HD05
            FlowPRA Single Antigen HLA Class I Supplement - Group 5
            HLA-B
            10 tests
            Full Specification
            HLA Class I
            Product Name FlowPRA Single Antigen HLA Class I Supplement - Group 5
            Format HLA-B
            Regulatory For In Vitro Diagnostic Use.
            Use To identify HLA Class I antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of B locus, and one control bead that is not coated with antigen.
            Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
            Shipping Considerations Dry ice
            Storage Temperature -80° to -65° C or below
            Amount 10 tests
            Detection Method Flow Cytometric
            Technique ELISA
            Typing Resolution High
            Target Antigens: B*37:01, B*39:01, B*41:01, B*42:01, B846:01, B*47:01, B*48:01, B*50:01

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              FL1HD06
              FlowPRA Single Antigen HLA Class I Supplement - Group 6
              HLA-B
              10 tests
              Full Specification
              HLA Class I
              Product Name FlowPRA Single Antigen HLA Class I Supplement - Group 6
              Format HLA-B
              Regulatory For In Vitro Diagnostic Use.
              Use To identify HLA Class I antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of B locus, and one control bead that is not coated with antigen.
              Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
              Shipping Considerations Dry ice
              Storage Temperature -80° to -65° C or below
              Amount 10 tests
              Detection Method Flow Cytometric
              Technique ELISA
              Typing Resolution High
              Target Antigens: B*14:01, B*15:16, B*40:02, B*53:01, B*54:01, B*56:01, B*58:01, B*59:01

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                FL1HD07
                FlowPRA Single Antigen HLA Class I Supplement - Group 7
                HLA-B
                10 tests
                Full Specification
                HLA Class I
                Product Name FlowPRA Single Antigen HLA Class I Supplement - Group 7
                Format HLA-B
                Regulatory For In Vitro Diagnostic Use.
                Use To identify HLA Class I antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of B locus, and one control bead that is not coated with antigen.
                Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
                Shipping Considerations Dry ice
                Storage Temperature -80° to -65° C or below
                Amount 10 tests
                Detection Method Flow Cytometric
                Technique ELISA
                Typing Resolution High
                Target Antigens: B*15:02, B*15:03, B*15:12, B*15:13, B*76:01, B*73:01, B*78:01, B*81:01

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                  FL1HD08
                  FlowPRA Single Antigen HLA Class I Supplement - Group 8
                  HLA-A, B
                  10 tests
                  Full Specification
                  HLA Class I
                  Product Name FlowPRA Single Antigen HLA Class I Supplement - Group 8
                  Format HLA-A, B
                  Regulatory For In Vitro Diagnostic Use.
                  Use To identify HLA Class I antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of A and B loci, and one control bead that is not coated with antigen.
                  Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
                  Shipping Considerations Dry ice
                  Storage Temperature -80° to -65° C or below
                  Amount 10 tests
                  Detection Method Flow Cytometric
                  Technique ELISA
                  Typing Resolution High
                  Target Antigens: A*36:01, A*43:01, A*66:01, A*69:01, A*74:01, A*80:01, B*15:10, B*82:01

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                    FL1HD09
                    FlowPRA Single Antigen HLA Class I Supplement - Group 9
                    HLA-C
                    10 tests
                    Full Specification
                    HLA Class I
                    Product Name FlowPRA Single Antigen HLA Class I Supplement - Group 9
                    Format HLA-C
                    Regulatory For In Vitro Diagnostic Use.
                    Use To identify HLA Class II antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 different beads coated with purified single antigens for a panel typing of C locus, and one control bead that is not coated with antigen.
                    Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
                    Shipping Considerations Dry ice
                    Storage Temperature -80° to -65° C or below
                    Amount 10 tests
                    Detection Method Flow Cytometric
                    Technique ELISA
                    Typing Resolution High
                    Target Antigens: C*01:02, C*02:02, C*03:02, C*04:01, C*05:01, C*06:02, C*07;02

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                      FL1HD10
                      FlowPRA Single Antigen HLA Class I Supplement - Group 10
                      HLA-C
                      10 tests
                      Full Specification
                      HLA Class I
                      Product Name FlowPRA Single Antigen HLA Class I Supplement - Group 10
                      Format HLA-C
                      Regulatory For In Vitro Diagnostic Use.
                      Use To identify HLA Class I antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of C locus, and one control bead that is not coated with antigen.
                      Package Includes 1 vial FlowPRA Single Antigen Class I beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
                      Shipping Considerations Dry ice
                      Storage Temperature -80° to -65° C or below
                      Amount 10 tests
                      Detection Method Flow Cytometric
                      Technique ELISA
                      Typing Resolution High
                      Target Antigens: C*08:01, C*12:03, C*14:02, C*15:02, C*16:01, C17:01, C*18:02, C*03:04

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                        FL2HD
                        FlowPRA Single Antigen HLA Class II
                        HLA-DR, DQ, DP
                        10 tests
                        Full Specification
                        HLA Class II
                        Product Name FlowPRA Single Antigen HLA Class II
                        Format HLA-DR, DQ, DP
                        Regulatory For In Vitro Diagnostic Use.
                        Use To identify HLA Class II IgG antibody specificities in high PRA sera by flow cytometry. A panel composed of 32 microbeads coated with purified HLA Class I single or fractional antigens divided into 4 groups for a panel typing of DRB1, DRB3, DRB4, DRB5, and DQB1loci.
                        Package Includes 4 vials (Groups, 1, 2, 3. 4) FlowPRA Single Antigen Class II Beads (Cat.# FL2HDBD1, FL2HDBD2, FL2HDBD3, FL2HDBD4) 60 µl vials each vial, 5 µl per test, 10X wash buffer (Cat.# FL-BF) 26 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-AB) 60 µl vial
                        Shipping Considerations Dry ice
                        Storage Temperature -80° to -65° C or below
                        Amount 10 tests
                        Detection Method Flow Cytometric
                        Technique ELISA
                        Typing Resolution High
                        Target Group 1: DRB1*01:01, DRB1*01:03, DRB1*04:01, DRB1*04:05, DRB1*07:01, DRB1*08:01, DRB1*10:01, DRB1*11:01
                        Group 2: DRB1*03:01, DRB1*03:02, DRB1*12:01, DRB1*13:01, DRB1*13:03, DRB1*14:01, DRB1*15:01, DRB1*16:01
                        Group 3: DRB1*01:02, DRB1*04:04, DRB1*09:01, DRB1*12:02, DRB1*15:02, DRB3*02:02, DRB4*01:03, DRB5*01:01
                        Group 4: DQB1*02:01, DQB1*03:01, DQB1*03:02, DQB1*03:03, DQB1*04:02, DQB1*05:01, DQB1*06:02, DPB1*01:01,02:01,03:01,04:01,05:01,11:01,13:01,17:01

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                          FL2HD01
                          FlowPRA Single Antigen HLA Class II - Group 1
                          HLA-DR
                          10 tests
                          Full Specification
                          HLA Class II
                          Product Name FlowPRA Single Antigen HLA Class II - Group 1
                          Format HLA-DR
                          Regulatory For In Vitro Diagnostic Use.
                          Use To identify HLA Class II antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 microbeads coated with purified single antigens for a panel typing of DRB1, and one control bead that is not coated with antigen.
                          Package Includes 1 vial FlowPRA Single Antigen Class II beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
                          Shipping Considerations Dry ice
                          Storage Temperature -80° to -65° C or below
                          Amount 10 tests
                          Detection Method Flow Cytometric
                          Technique ELISA
                          Typing Resolution High
                          Target Antigens: DRB1*01:01, DRB1*01:03, DRB1*04:01, DRB1*04:05, DRB1*07:01, DRB1*08:01, DRB1*10:01, DRB1*11:01

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                            FL2HD02
                            FlowPRA Single Antigen HLA Class II - Group 2
                            HLA-DR
                            10 tests
                            Full Specification
                            HLA Class II
                            Product Name FlowPRA Single Antigen HLA Class II - Group 2
                            Format HLA-DR
                            Regulatory For In Vitro Diagnostic Use.
                            Use To identify HLA Class II antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 different beads coated with purified single antigens for a panel typing of DRB1, and one control bead that is not coated with antigen.
                            Package Includes 1 vial FlowPRA Single Antigen Class II beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
                            Shipping Considerations Dry ice
                            Storage Temperature -80° to -65° C or below
                            Amount 10 tests
                            Detection Method Flow Cytometric
                            Technique ELISA
                            Typing Resolution High
                            Target Antigens: DRB1*03:01, DRB1*03:02, DRB1*12:01, DRB1*13:01, DRB1*13:03, DRB1*14:01, DRB1*15:01, DRB1*16:01

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                              FL2HD03
                              FlowPRA Single Antigen HLA Class II - Group 3
                              HLA-DR
                              10 tests
                              Full Specification
                              HLA Class II
                              Product Name FlowPRA Single Antigen HLA Class II - Group 3
                              Format HLA-DR
                              Regulatory For In Vitro Diagnostic Use.
                              Use To identify HLA Class II antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 different beads coated with purified single antigens for a panel typing of DRB1/DRB3,4,5, and one control bead that is not coated with antigen.
                              Package Includes 1 vial FlowPRA Single Antigen Class II beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
                              Shipping Considerations Dry ice
                              Storage Temperature -80° to -65° C or below
                              Amount 10 tests
                              Detection Method Flow Cytometric
                              Technique ELISA
                              Typing Resolution High
                              Target Antigens: DRB1*01:02, DRB1*04:04, DRB1*09:01, DRB1*12:02, DRB1*15:02, DRB3*02:02, DRB4*01:03, DRB5*01:01

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                                FL2HD04
                                FlowPRA Single Antigen HLA Class II - Group 4
                                HLA-DQ, DP
                                10 tests
                                Full Specification
                                HLA Class II
                                Product Name FlowPRA Single Antigen HLA Class II - Group 4
                                Format HLA-DQ, DP
                                Regulatory For In Vitro Diagnostic Use.
                                Use To identify HLA Class II antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 different beads coated with purified single antigens for a panel typing of DQB1 locus antigens, and one control bead that is not coated with antigen.
                                Package Includes 1 vial FlowPRA Single Antigen Class II beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
                                Shipping Considerations Dry ice
                                Storage Temperature -80° to -65° C or below
                                Amount 10 tests
                                Detection Method Flow Cytometric
                                Technique ELISA
                                Typing Resolution High
                                Target Antigens: DQB1*02:01, DQB1*03:01, DQB1*03:02, DQB1*03:03, DQB1*04:02, DQB1*05:01, DQB1*06:02,DPB1*01:01,02:01,03:01,04:01,05:01,11:01,13:01,17:01

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                                  FL2HD05
                                  FlowPRA Single Antigen HLA Class II Supplement - Group 5
                                  HLA-DP
                                  10 tests
                                  Full Specification
                                  HLA Class II
                                  Product Name FlowPRA Single Antigen HLA Class II Supplement - Group 5
                                  Format HLA-DP
                                  Regulatory For In Vitro Diagnostic Use.
                                  Use To identify HLA Class II antibody specificities in high PRA sera by flow cytometry. A panel composed of 8 different beads coated with purified single antigens for a panel typing of DPB1 locus, and one control bead that is not coated with antigen.
                                  Package Includes 1 vial FlowPRA Single Antigen Class II beads (60 µl vials each vial, 5 µl per test), 10X wash buffer (Cat.# FL-BFSUP) 7 ml, and 100X FITC conjugated F(ab')2 anti-human IgG (Cat.#FL-ABSUP) 20 µl vial
                                  Shipping Considerations Dry ice
                                  Storage Temperature -80° to -65° C or below
                                  Amount 10 tests
                                  Detection Method Flow Cytometric
                                  Technique ELISA
                                  Typing Resolution High
                                  Target Antigens: DPB1*01:01, DPB1*02:01, DPB1*03:01, DPB1*04:01, DPB1*05:01, DPB1*11:01, DPB1*13:01, DPB1*17:01

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