How Does LMT Work?
Viable lymphocytes are incubated with a mixture of complement-binding monoclonal antibodies and complement. If the lymphocytes express an antigen recognized by a specific antibody, the Fab portion of the antibody binds to the antigen, forming an antigen-antibody complex. After these complexes have formed, the C1q and Ca++ from the complement binds to the FC portion of the antibody. One IgM antibody is required to bind one molecule of C1q or two IgG antibodies are required to bind one molecule of C1q. Binding of C1q initiates the complement cascade, which leads to cell lysis with antigen antibody complexes. In a negative reaction, the lymphocytes remain viable. In a positive reaction, the lymphocytes are dead.